Improved early development potence of in vitro fertilization embryos by treatment with tubacin increasing acetylated tubulin of matured porcine oocytes
To enhance the developmental potential of in vitro embryos is really a lengthy-term concern field for human aided reproduction and animal in vitro embryo production practice. In the present study, we examined the results and mechanism of the HDAC6 inhibitor, tubacin, around the maturation of porcine oocytes as well as in vitro growth and development of porcine In vitro fertilization treatments embryos. It’s been shown the result of tubacin around the acetylation degree of a-tubulin in porcine oocytes. Consequently, the maturation rate of porcine oocytes was considerably improved (P < 0.05), and the following development potent of blastocysts forming rate was also significantly increased (P < 0.05). We found that the increased acetylation of a-tubulin significantly reduced the abnormal rate of microtubule, furthermore, the proportion of mitochondria in the vicinity of in vitro fertilization (IVF) nucleus was significantly enhanced in Metaphase I (MI) and Metaphase II (MII) stages. The expression Tubacin levels of microtubule assembly genes (TUBA1A, aTAT1 and MAP2) significantly up-regulated in MI and MII stages. Together, these results suggest that treatment of porcine oocytes during maturation with tubacin could promote their IVF embryos developmental competence by altering spindle formation, mitochondrial concentration and genes expression patterns of matured porcine oocytes